Composite

Part:BBa_K5349008:Design

Designed by: Tomoharu Sakata   Group: iGEM24_BotchanLab-Tokyo   (2024-09-26)


HSV/TK GCV system , UL23::SR39


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 397
    Illegal PstI site found at 1068
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 397
    Illegal PstI site found at 1068
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1611
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 397
    Illegal PstI site found at 1068
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 397
    Illegal PstI site found at 1068
    Illegal NgoMIV site found at 879
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For cloning, overlap sequences were designed for each fragment, and the assembly was performed using NEBuilder. Each fragment was synthesized using Integrated DNA Technologies' DNA synthesis service.


Source

  • promotor:
  • RBS:[ B0034]
  • Sbm operon
  • sfGFP
  • terminator

References